Formulation and Evaluation of Gel of Binahong Leaves Nanoparticle Extract (Anredera cordifolia (Ten.) Steenis) as an Antioxidant and Sunscreen

Fathiyah Ghina; Ro’ufi Absor; Deni Rahmat

doi:10.58511/v3i1.9216

Penulis

Fathiyah Ghina Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Pancasila, Jakarta, Indonesia
Ro’ufi Absor Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Pancasila, Jakarta, Indonesia
Deni Rahmat Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Pancasila, Jakarta, Indonesia

DOI:

https://doi.org/10.58511/v3i1.9216

Kata Kunci:

Binahong Leaves, Nanoparticle, Gel, Antioxidant, Sunscreen

Abstrak

Binahong leaves are a herbal plant that is used to cure several diseases in Indonesia. Binahong leaves have flavonoids that can be potentially beneficial for antioxidants and sunscreen. The study aims to formulate an extract and a nanoparticle extract from binahong leaves into a gel and determine antioxidant and sunscreen activity. Binahong leaves were macerated using 70% ethanol and concentrated with a rotary evaporator. Then the extract was made into nanoparticles using the ionic gelation method. The nanoparticles were characterized for particle size, zeta potential, and morphological shape. The results showed that the nanoparticles were 192.7 nm in size, zeta potential of +25.3 mV, and spherical in shape. Antioxidant and sunscreen activity tests were analyzed using a UV-Vis spectrophotometer. Determination of antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenger activity method. The binahong leaves extract and nanoparticles had IC50 values of 68.78 ppm (moderate) and 152.18 ppm (weak). The antioxidant gel binahong leaves had IC50 of Formula II (174.85 ppm) and Formula III (221.14 ppm). The sunscreen activity was analyzed by determining the SPF value. Binahong leaves extract and nanoparticles had SPF values of 16.92 and 21.55 (ultra category). The sunscreen gel binahong leaves extract (formula II) and nanoparticles (formula III) had SPF values of 2.61 and 3.3 (minimal category). Gel preparations were evaluated, including organoleptic, homogeneity, spreadability, viscosity, and pH test. Binahong leaves extract and nanoparticles extract had antioxidant activity and SPF value that could be formulated into a gel preparation.

Referensi

[1] Karina N. Penentuan Nilai Sun Protection Factor (SPF) Ekstrak dan Fraksi Rimpang Lengkuas (Alpinia galanga) sebagai Tabir Surya dengan Metode Spektrofotometer UV-Vis. Pontianak: Prodi Farmasi Fakultas Kedokteran Universitas Tanjungpura. 2015.

[2] Lung JKS, Dika PD. Review Artikel: Uji Aktivitas Antioksidan Vitamin A, C, E dengan Metode DPPH. Farmaka: Suplemen. 2017; 15(1): 54.

[3] Tahar N, Infriani N, Nonci FY. Efek tabir surya ekstrak daun binahong (Anredera cordifolia). ad-Dawaa’J.Pharm.Sci. 2019; 2(1): 29-35.

[4] Gunarti NS, Fikayuniar L. Formulasi dan uji aktivitas gel tabir surya dari ekstrak buah blackberry (Rubus fruticosus) secara in vitro dengan spektrofotometri uv-visible. Kartika: Jurnal Ilmiah Farmasi. 2019; 7(1): 66-72.

[5] Regina, A P dkk. Formulasi Nanopartikel Ekstrak Buah Parijoto (Medinilla speciosa Blume) dengan Variasi Enkapsulan Kitosan, Alginat dan Natrium Tripolifosfat. Indonesian Journal of Pharmacy and Natural Products. Vol 02, No. 01; 2019.

[6] Abdassah M. Nanopartikel dengan gelasi ionik. Farmakal;1. 15(1):45-52. 2017.

[7] Gazori T, Khoshayand MR, Azizi E, Yazdizade P, Nomani A, Haririan I. Evaluation of Alginate/Chitosan nanoparticles as antisense delivery vector: Formulation, optimization and in vitro characterization. Carbohydrate Polymers. 2009; 77(3): 599-606.

[8] Wasitaatmadja SM, Menaldi SL. Peremajaan Kulit. Jakarta: Penerbit UI-Press; 2003, h 1-9, 119-120.

[9] Martien R, Adhyatmika, Irianto LD. Farida V, Sari DP. Technology Development of Nanoparticles as Drugs. Majalah Farmaseutik. 2012; 8(1): 133-144.

[10] Murti GPK. Pengaruh pH terhadap Sifat Fisik dan Stabilitas Fisik Gel pada Gel Ekstrak Etanolik Daun Sirih Merah (Piper crocatum Ruiz. & Pav.) dengan Kombinasi Gelling Agent Karbopol dan Carboxymethylcellulose Sodium (CMC-Na) (skripsi). Yogyakarta: Universitas Gadjah Mada; 2017.

[11] Yulitah D, Dewi R, Krisman. Fabrikasi dan Karakterisasi Sifat Optik dari Ba1-xSrxTiO3 Menggunakan Spektrofotometer Ultraviolet Visible. JOM MIPA. 2015; 2(1): 136.