Determination of the Content of Flavonoids and Total Phenols in Film Strips of 70% Ethanol Extract of Asam Gelugur Leaves (Garcinia atroviridis Griff. ex T. Anderson) using the Colorimetric Method
DOI:
https://doi.org/10.58511/jnpdd.v4i1.10359Kata Kunci:
Garcinia atroviridis Griff. ex T. Anderson, Film Strip, Total Flavonoid, Total Phenolic, SpektrophotometryAbstrak
Asam gelugur leaves contain phenolic compounds or polyphenols that act as natural antioxidants with anti-hyperlipidaemic activity, such as flavonoids and phenols. The aim of this study was to produce 70% ethanol extract film strips from asam gelugur leaves that meet the criteria for evaluation of physical, chemical and microbiological quality parameters, and to establish an analytical method for determination flavonoid and phenol content. The research method employed a colorimetric approach using a microplate reader. The results of this study yielded thin-film strip preparations characterised by a dark brown colour, a distinctive odour, a minty and sour taste, a disintegration time of 285 ± 8.50 seconds, a moisture content of 1.62–2.42 per cent, a weight uniformity of 0.4565, film thickness of 0.13 mm, a pH range of 2.53–2.68, no microbial contamination detected in week 0 and a count of 0.2768 × 10⁴ in week 4, the average total flavonoid content in the extract was 3.3954 mg QE/g extract and in the film strips 1.8936 mg QE/g extract, and the average total phenolic content in the extract was 23.4090 mg GAE/g extract and in the film strips 13.8111 mg GAE/g extract. The conclusion of this study is that the film strips did not meet the requirements for several quality evaluation parameters, and the flavonoid and phenolic content levels were higher in the extract than in the film strips.
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